Circular Dichroism Test Chemistry - Hydrolysis of Glucuronolactone
The lactone structure of glucuronolactone gives the molecule its chiral properties. On mixing glucuronolactone with potassium hydroxide, hydrolysis takes place causing the loss of the lactone structure. This results in a decreasing CD signal, as measured at 225nm, that decreases to zero in about 0.5s.
Sample Preparation
| Solution 1:- | 25 x 10 -3 M Glucuronolactone in water (4.4mg/mL) |
| Solute 1: | Glucuronolactone |
| Source: | Sigma G-8875 |
| MW: | 176.1 |
Prepare 4.4mg/mL glucuronolactone in water
| Solution 2:- | 1M potassium hydroxide |
| Solute 1: | Potassium hydroxide (KOH) |
| Source: | Sigma P-5958 |
| MW: | 56.11 |
Dissolve 14g potassium hydroxide in 250mL of distilled water.
Risk Assessment
Glucuronolactone may irritate the eyes.
Potassium hydroxide is an irritant to the eyes and skin and prolonged contact will cause burns.
Wash hands as a precaution after handling.
| Measurement Conditions | |
| Syringe Ratio: | 1:1 (both syringes 2.5mL) |
| Glucuronolactone conc. in cell: | 2.2mg/mL |
| Optical Path: | 2mm |
| Monochromator Wavelength: | 225nmm |
| Monochromator bandwidth: | 2nm |
| Acquisition period: | 0.5s |