Applied Photophysics Ltd

Chirascan™

SF.3 Stopped-flow accessory

The SF.3 is a dedicated stopped-flow accessory for the Chirascan circular dichroism spectrometer.

The SF.3 accessory brings to Chirascan our world-leading expertise in stopped-flow design to enable circular dichroism (CD), absorbance and fluorescence stopped-flow measurements of unsurpassed sensitivity. The unit is based on our best-selling SX20 and PiStar stopped-flows, and designed to enable rapid and straightforward coupling to Chirascan: a roller-plate mount enables it to be easily located and locked into place at the exit slit of the Chirascan monochromator (in place of the sample chamber unit).


A demountable stopped-flow cell provides optical pathlengths of 2mm and 10mm and has a dead-time of 1.5ms. Cells can be changed in a few minutes; a smaller, lower dead-time, cell is available as an option. Circular dichroism (CD) and absorbance kinetics are collected using the standard Chirascan detector. Fluorescence detection is also possible with option TF.3.


SF.3 Stopped-flow accessory


Single shot data examples showing circular dichroism, absorbance and fluorescence stopped-flow kinetic traces.


Chirascan circular dichroism spectromter with SF.3 accessory
Chirascan Circular Dichroism Spectrometer with SF.3 stopped-flow accessory

The SF.3 stopped-flow accessory is easily mounted to the Chirascan.

Accessory Features

  • Optimized for circular dichroism, absorbance and fluorescence detection without the need for reconfiguration.
  • Straightforward benchtop configuration. Can be fitted in 10 minutes.
  • Low sample volume requirement.
  • Flow circuit materials suitable for anaerobic experiments and aggressive reagents.
  • Short optical pathlength for fluorescence detection (minimizes inner-filtering effect).
  • Large ratio-mixing capability up to 25:1
  • Comprehensive range of accessories available including sequential-mixing (SQ.3), fluorescence polarization (FP.3) and dual fluorescence detection (DF.3).

Detailed specification for the SF.3 stopped-flow accessory is available here.

A quick introduction to stopped-flow is available here.


Other Chirascan Accessories


Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer
Chirascan Circular Dichroism Spectrometer