SX20 Stopped-Flow Spectrometer Accessories - Pro-K II Global Analysis
Pro-K II Global Analysis
This is a unique stand-alone software package providing global analysis of time resolved spectral data accompanied by a comprehensive data simulation capability. It features singular value decomposition (a type of factor analysis) allowing spectral component prediction and data reduction. Global fitting to an arbitrary reaction schemes is available through the use of fast numerical integration techniques coupled to a symbolic reaction scheme editor. True intermediate spectra of all coloured species are calculated together with their associated concentration profiles. Multi-wavelength kinetic and PDA data are ideally suited for analysis with Pro-K.
ProK II has all of the functionality of PC ProK and in addition allows the simultaneous analysis of multiple datasets gathered at different sets of initial conditions (usually concentration). This capability is unique and greatly increases the ability to resolve rates and intermediate spectra of more complex reaction mechanisms directly from stopped-flow data. The simplest example is the 2nd order reaction: A+B>C. Standard global analysis packages (including PC ProK) cannot directly determine the spectra of A and B from analysis of a multi-wavelength data set as they are linearly dependant; it is necessary to fix the spectrum of one of the reactants. However, by performing the reaction at two (or more) starting concentrations of A and B and submitting all of the datasets to ProK II, the spectra and the rate can be directly obtained. A more complex example is the reaction: A+B<>C<>D, where all spectra and all forward and reverse rates can be determined using ProK II.
Pro-K II includes powerful 3D graphing capabilities
Other features of ProK II include:
- Singular value decomposition for spectral component prediction and random noise reduction.
- The use of numerical integration which places virtually no limit on the complexity of the reaction mechanism used for fitting.
- A powerful data simulator allowing the user to investigate reaction models, rates and spectral parameters. Random data noise can also be simulated enabling the user to explore the analysis (fitting) capabilities of typical ‘idealised’ data with the reaction model. The simulation tool can also be used to gain familiarity with the package.
- Mixed data types: simultaneous analysis of, for example, fluorescence and absorbance data to further aid the elucidation of reaction rates and mechanisms.
- New support for rapid equilibrium and protonation steps in the reaction model.
- Comprehensive visualisation tools for inspecting results.
Pro-K II is also available as a free 30 day try-before-you-buy basis. Contact us for further details.
Click here for an introduction to global analysis.
Other SX20 Accessories
- SQ.1 Sequential mixing
- PDA.1 Photodiode array
- SEM.1 Scanning Emission Monochromator
- AM.1 Scanning Monochromator (far-UV)
- UV.1 Deuterium light source
- RC5.1 5µl cell cartridge
- FP.1 Fluorescence polarisation/anisotropy
- DD.1 Dual detection accessory
- DF.1 Dual fluorescence detection accessory
- AN.1 Anaerobic system
- QFA.1 Quench-flow adaptor
- Pro-K Global analysis for PC
- Pro-K II 2 nd Order Global analysis for PC
Click here to request a full PDF brochure for the SX20 Stopped-Flow Spectrometer and accessories.
SX20 - the ultimate gold standard in stopped-flow
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